Hello,
I am wanting to accurately and robustly segment both nucleus and cells in colorectal tissue using Tissue studio 3. Is H&E good enough for this or will I need a specific membrane/whole cell stain? Is there stains I can multiplex with H&E for this or is IHC the way forward along with a nuclear dye e.g.DAPI? As I am interested in stroma, healthy and tumour cells cytokeratin alone would not be suitable.
Many thanks,
Peter
Tissue staining for accurate cell segmentation
Date: 12/07/2011 [02:17 PM]
Date: 12/09/2011 [11:46 AM]
Hello Peter,
If you would like to have exact cell boundaries, we recommend using a membrane IHC stain for use with Tissue Studio. In other cases, you can use the Cell Simulation function, which can either be based on a fixed distance from the nucleus or based on a whole-cell stain.
I am not aware of any customer that has used a reliable stain cocktail for multiple cell types (stroma, healthy and tumor cells). Maybe other users can comment?
Best wishes,
Kai
If you would like to have exact cell boundaries, we recommend using a membrane IHC stain for use with Tissue Studio. In other cases, you can use the Cell Simulation function, which can either be based on a fixed distance from the nucleus or based on a whole-cell stain.
I am not aware of any customer that has used a reliable stain cocktail for multiple cell types (stroma, healthy and tumor cells). Maybe other users can comment?
Best wishes,
Kai