Hello,
I was wondering if anyone had differentiated colorectal stroma from healthy epithelial from dysplastic (cancer) epithelial using H&E staining alone in TS3? Can this be done by teaching the programme to recognise these ROIs using the composer software?
If H&E is not specific enough would you need to apply a cytokeratin stain?
Thanks,
Peter
H&E alone Vs cytokeratin for tumour detection
Date: 12/07/2011 [02:45 PM]
Date: 12/09/2011 [11:53 AM]
Hello Peter,
You can use a 2-step approach: a combination of Composer ROI detection with the Nucleus Morphology Filter, which is new in Tissue Studio 3.0. This could give you a good distinction already on H&E between healthy and dysplastic cells. I would expect that there will be border-line nuclei between the two classes (healthy and dysplastic) that can not be judged by the morphology filter - mainly because the morphology is almost identical.
A cytokeratin stain could be used to improve the result of the initial Composer ROI detection, but would be of no effect to the second step.
Best wishes,
Kai
You can use a 2-step approach: a combination of Composer ROI detection with the Nucleus Morphology Filter, which is new in Tissue Studio 3.0. This could give you a good distinction already on H&E between healthy and dysplastic cells. I would expect that there will be border-line nuclei between the two classes (healthy and dysplastic) that can not be judged by the morphology filter - mainly because the morphology is almost identical.
A cytokeratin stain could be used to improve the result of the initial Composer ROI detection, but would be of no effect to the second step.
Best wishes,
Kai