Overview

Customized algorithm for rounding obejct variables to x decimal places.

Customized algorithm for rounding scene variables to x decimal places.

Another method to implement the computation of the correlation coefficient for quantification of co localizations is shown. The relative brightness in red and green image channel is calculated with...

Quick method to implement the correlation coefficient for quantification of colocalizations

We demonstrate how to bring together several images using customized import, yielding a scene a) containing the images as layers or b) containing the images as maps

Artificial timeseries to explore some more interesting object linking scenarios.

Technical example on how to create multiple 3D objects for each slice of an object

This example shows how to expand seeds dependent on the individual seed characteristics (local conditions) using pixel-based object resizing instead of introducing an object neighborhood and using...

Allows the detection of transition zones between dark and bright regions independently of absolute intensities.

An easy approach allowing automatic threshold computation on a restricted value range.

The rule set presented writes the brightest pixel of all slices in a stack into an additional image layer.

Creates a temporary layer with the density of objects of a certain class as intensity information.

A distance map is a powerful layer to measure for every pixel in the object the minimal distance to the objects border. Here we use a distance map to compute distances between nuclei and estimate...

Fast alternative to 'Merge' command based on XD algorithms

Calculate the amount of stain contained in another stain

Object ids are used in many rule sets. You can use a sorted image object list to give object ids to objects in a structured way.

This short demo shows several things:

1) How to calculate a layer which contains the distance to a line with the process "layer arithmetics". This serves as a demo case on how to use mathematical...

These lines of code create two layers that represent X and Y values of a coordinate system that has been shifted and pivoted to fit to a given structure.

The novel algorithm "Polygon Cut" in release 1.5 of Definiens Developer allows the straightforward implementation of annotations and manual measurements in interactive workflows